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Marker assisted backcrossing Workflow

Marker assisted backcrossing (MABC): Introgression of one or more marked segments for a donor to a recipient germplasm using backcrossing and marker selection

Activity
Logistical Tools
Analytic Tools
Assuming identified and validated trait markers (usually qualitative traits) select donor and recipient material. Plan crosses.

MBDT: analysis based on target genotype, genetic distance to recipient germplasm, adaptation and potential adoption.
Planting hybridization block, make crosses
SETGEN: Sample tracking and pedigree management
INTRACK: Seed inventory management

Make first backcross, genotype BC1F1s
SETGEN: Sample tracking, bar coding and LIMS. Data loading to breeding databases.
INTRACK: Seed inventory management.
MOSEL: select plants heterozygous for donor alleles at target loci, minimize size of introgression and select lines homozygous recipient or heterozygous at background loci.
Backcross selected plants to recipient, repeat genotyping and selection
SETGEN: Sample tracking, pedigree management, bar coding and LIMS. Data loading to breeding databases.
MOSEL
Possibly self selected plants to increase homozygosity
SETGEN: Sample tracking and pedigree management.
INTRACK: Seed inventory management.
MOSEL: minimize introgression size and fix critical markers
Repeat previous two steps until selected lines are sufficiently homozygous for background and can be easily made homozygous for target loci.
SETGEN: Sample tracking, pedigree management, bar coding and LIMS. Data loading to breeding databases.
INTRACK: Seed inventory management.
MOSEL
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